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2022

  • Wan-Wen Ting, I-Son Ng* (2022) Effective 5-aminolevulinic acid production via T7 RNA polymerase and RuBisCO equipped Escherichia coli W3110. Biotechnology Bioengineering, 120(2), 583-592. DOI: 10.1002/bit.28273

  • Chengfeng Xue, I‐Son Ng* (2022) A direct enzymatic evaluation platform (DEEP) to fine‐tuning pyridoxal 5’‐phosphate‐dependent proteins for cadaverine production. Biotechnology Bioengineering, 120(1), 272-283. DOI: 10.1002/bit.28270

  • Zengling Ma, Wai Yan Cheah, I-Son Ng, Jo-Shu Chang*, Min Zhao*, Pau Loke Show* (2022) Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions. Trends in Biotechnology, 40, 1439-1453.

  • Wan-Wen Ting, Jie-Yao Yu, Yu-Chieh Lin, I-Son Ng* (2022) Enhanced recombinant carbonic anhydrase in T7RNAP-equipped Escherichia coli W3110 for carbon capture storage and utilization (CCSU). Bioresource Technology, 363, 128010.

  • Sefli Sri Wahyu Effendi, Jia-Yi Lin, I-Son Ng * (2022) Simultaneous carbon dioxide sequestration and utilization for cadaverine production using dual promoters in engineered Escherichia coli strains. Bioresource Technology, 363, 127980.

  • Sefli Sri Wahyu Effendi, I-Son Ng* (2022) Reprogramming T7RNA polymerase in Escherichia coli Nissle 1917 under specific lac operon for efficient p‑coumaric acid production. ACS Synthetic Biology, 11, 3471-3481.

  • Sefli Sri Wahyu Effendi, I-Son Ng* (2022) Prospective and challenges of live bacterial therapeutics from a superhero Escherichia coli Nissle 1917, Critical Reviews in Microbiology, 49(5), 611-627. DOI: 10.1080/1040841X.2022.2109405

  • Yi-Lun Huang, Yu-Ling Hsu, Ya-Chu Yu, Hong-Yan Huang, Ren-Hao Tsai, Yao-Tien Cheng, Yi-Ling Chou, Sheng-Yan Sun, Li-An Wang, Jia-Yi Lin, Chao-Chung Chen, Jia-Horung Hung*, I-Son Ng* (2022) A systematic approach to reduce intraocular pressure (IOP) for the treatment of glaucoma. Biotechnology Progress, 38(6):e3285. DOI: 10.1002/btpr.2834

  • Chuan-Chieh Hsiang, Priskila Adjani Diankristanti, Shih-I Tan, Yi-Chia Ke, Yeong-Chang Chen, Sefli Sri Wahyu Effendi, I-Son Ng* (2022) Tailoring key enzymes for renewable and high-level itaconic acid production using genetic Escherichia coli via whole-cell bioconversion. Enzyme and Microbial Technology, 160, 110087.

  • Yen-Ju Lee, Ying-Chen Yi, Yu-Chieh Lin, Chao-Chung Chen, Jia-Horung Hung, Jia-Yi Lin, I-Son Ng* (2022) Purification and biofabrication of 5-aminolevulinic acid for photodynamic therapy against pathogens and cancer cells. Bioresource and Bioprocessing, 9:68.

  • Shih-Chen Yang, Wan-Wen Ting, I-Son Ng* (2022) Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli. Biochemical Engineering Journal, 185, 108502.

  • Chiau-Sin Teng, Chengfeng Xue, Jia-Yi Lin, I-Son Ng* (2022) Towards high-level protein, beta-carotene, and lutein production from Chlorella sorokiniana using aminobutyric acid and pseudo seawater. Biochemical Engineering Journal, 184, 108473.

  • Jia-Yi Lin, Sefli Sri Wahyu Effendi, I-Son Ng* (2022) Enhanced carbon capture and utilization (CCU) using heterologous carbonic anhydrase in Chlamydomonas reinhardtii for lutein and lipid production. Bioresource Technology, 351, 127009.

  • Jia-Yi Lin, Shih-I Tan, Ying-Chen Yi, Chuan-Chieh Hsiang, Chien-Hsiang Chang, Chun-Yen Chen, Jo-Shu Chang, I-Son Ng* (2022) High-level production and extraction of C-Phycocyanin from Cyanobacteria Synechococcus sp. PCC7002 for antioxidation, antibacterial and lead adsorption. Environmental Research, 206, 112283.

  • Tsung-Han Tsai, Jia-Yi Lin, I-Son Ng* (2022) Cooperation of phytoene synthase, pyridoxal kinase and carbonic anhydrase for enhancing carotenoids biosynthesis in genetic Chlamydomonas reinhardtii. Journal of the Taiwan Institute of Chemical Engineers, 137, 104184.

  • Yu-Chieh Lin, Chengfeng Xue, Shih-I Tan, Wan-Wen Ting, Shih-Chen Yang, I-Son Ng* (2022) Precise measurement of decarboxylase and applied cascade enzyme for simultaneous cadaverine production with carbon dioxide recovery. Journal of the Taiwan Institute of Chemical Engineers, 137, 104188.

  • Tzu-Hsuan Yu, Shih-I Tan, Ying-Chen Yi, Chengfeng Xue, Wan-Wen Ting, Jui-Jen Chang, I-Son Ng* (2022) New insight into the codon usage and medium optimization toward stable and high-level 5-aminolevulinic acid production in Escherichia coli. Biochemical Engineering Journal, 177, 108259.

  • Chengfeng Xue, I-Son Ng* (2022) Sustainable production of 4-aminobutyric acid (GABA) and cultivation of Chlorella sorokiniana and Chlorella vulgaris as circular economy. Bioresource Technology, 343: 126089.

2021

  • Jia-Yi Lin#, Way-Rong Lin#, I-Son Ng* (2021) CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production. Biotechnology Journal, 20:e2100514.

  • Ying-Chen Yi, Chengfeng Xue, I-Son Ng* (2021) Low carbon footprint production of high-end 5-aminolevulinic acid via integrative strain engineering and RuBisCo-equipped Escherichia coli. ACS Sustainable Chemistry & Engineering, 9(46), 15623–15633.

  • Ying-Chen Yi, I-Tai Shih, Tzu-Hsuan Yu, Yen-Ju Lee, I-Son Ng* (2021) Challenges and opportunities of bioprocessing 5-aminolevulinic acid using geneticand metabolic engineering: a critical review. Bioresources and Bioprocessing, 8:13.

  • Shih-I Tan, Chuan-Chieh Hsiang, I-Son Ng* (2021) Tailoring genetic elements of plasmid-driven T7 system for stable and robust one-step cloning and protein expression in broad Escherichia coli. ACS Synthetic Biology, 10(2), 412-421. doi.org/10.1021/acssynbio.1c00014

  • Ya-Yun Chiang, Dillirani Nagarajana, Yung-Chung Lo, Chun-Yen Chen, I-Son Ng, Chien-Hsiang Chang, Duu-Jong Lee, Jo-Shu Chang*. (2021) Succinic acid fermentation with immobilized Actinobacillus succinogenes using hydrolysate of carbohydrate-rich microalgal biomass. Bioresource Technology, 342:126014.

  • Jia-Yi Lin, I-Son Ng* (2021) Production, isolation and characterization of C-phycocyanin from a new halo-tolerant Cyanobacterium aponinum using seawater. Bioresource Technology, 342:125946.

  • Sefli Sri Wahyu Effendi, Chengfeng Xue, Shih-I Tan, I-Son Ng* (2021) Whole-cell biocatalyst of recombinant tyrosine ammonia lyase with fusion protein and integrative chaperone in Escherichia coli for high-level p-Coumaric acid production. Journal of the Taiwan Institute of Chemical Engineers, 128, 64-72.

  • Chengfeng Xue, Ying-Chen Yi and I-Son Ng* (2021) Migration of glutamate decarboxylase by cold treatment on whole-cell biocatalyst triggered activity for 4-aminobutyric acid production in engineering Escherichia coli. International Journal of Biological Macromolecules 190, 113–119.

  • Shih-I Tan, I-Son Ng* (2021) Stepwise optimization of genetic RuBisCO-equipped Escherichia coli for low carbon-footprint protein and chemical production. Green Chemistry, 23, 4800-4813.

  • Wan-Wen Ting, Chih-Yu Huang, Pong-Yee Wu, Shih-Fang Huang, Hung-Yi Lin, Sheng-Feng Li, Jo-Shu Chang, I-Son Ng* (2021) Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of L-lysine decarboxylase in recombinant Escherichia coli W3110. Enzyme and Microbial Technology, 133:109458.

  • I-Tai Shih, Ying-Chen Yi, I-Son Ng* (2021) Plasmid-free system and modular design for efficient 5-aminolevulinic acid production by engineered Escherichia coli. Applied Biochemistry and Biotechnology 193(9), 2858–2871.
    https://doi.org/10.1007/s12010-021-03571-3

  • Chengfeng Xue, Tzu-Hsuan Yu, I-Son Ng* (2021) Engineering pyridoxal kinase PdxY-integrated Escherichia coli strain and optimization for high-level 5-aminolevulinic acid production. Journal of the Taiwan Institute of Chemical
    Engineers    , 120, 49-58.

  • Shih-I Tan, I-Son Ng* (2021) CRISPRi-mediated NIMPLY logic gate for fine-tuning the whole-cell sensing toward simple urine glucose detection. ACS Synthetic Biology, 10(2), 412-421. doi.org/10.1021/acssynbio.1c00014

  • Yi-Jun Lan, Shih-I Tan, Shu-Yun Cheng, Wan-Wen Ting, Chengfeng Xue, Tzu-Han Lin, Ming-Zhi Cai, Po-Ting Chen**, I-Son Ng* (2021) Development of Escherichia coli Nissle 1917 derivative by CRISPR/Cas9 and application for gamma-aminobutyric acid (GABA) production in antibiotic-free system. Biochemical Engineering Journal, 168, 107952.

  • Ying-Chen Yi, I-Son Ng* (2021) Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid production. Bioresources and Bioprocessing, 8:13.

  • Jia-Yi Lin, Chengfeng Xue, Shih-I Tan, I-Son Ng* (2021) Pyridoxal kinase PdxY mediated carbon dioxide assimilation to enhance the biomass in Chlamydomonas reinhardtii CC-400. Bioresource Technology, 322:124530.

  • Chengfeng Xue, Kai-Min Hsu, Chen-Yaw Chiu, Yu-Kaung Chang, I-Son Ng* (2021) Fabrication of bio-based polyamide 56 and antibacterial nanofiber membrane from cadaverine. Chemosphere, 266, 128967.

  • Sefli Sri Wahyu Effendi, Shih-I Tan, Wan-Wen Ting, I-Son Ng* (2021) Genetic design of co-expressed Mesorhizobium loti carbonic anhydrase and chaperone GroELS to enhancing carbon dioxide sequestration. International Journal of
    Biological Macromolecules    , 167, 326-334.

  • Sefli Sri Wahyu Effendi, Shih-I Tan, Wan-Wen Ting, I-Son Ng* (2021) Enhanced recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase activity and thermostability by chaperone GroELS for carbon dioxide
    biomineralization. Chemosphere, 271, 128461.

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